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Induction of a senescent-like phenotype does not confer the ability of bovine immortal cells to support the development of nuclear transfer embryos.

Biology of reproduction - 1 Jul 2003

Shi Wei, Hoeflich Andreas, Flaswinkel Heinrich, Stojkovic Miodrag, Wolf Eckhard, Zakhartchenko Valeri

Abstract excerpt

Previously, we reported that cloned embryos derived from an immortalized bovine mammary epithelial cell line (MECL) failed to develop beyond 12- to 16-cell stage. To analyze whether induction of a senescent-like phenotype in MECL can improve their ability to support the development after transfer into enucleated oocytes, we treated MECL with DNA methylation inhibitor 5-aza-2-deoxycytidine (Aza-C), histone...

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PMID: 12646489
DOI: 10.1095/biolreprod.102.012112

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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