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Adult cloning in cattle: Potential of nuclei from a permanent cell line and from primary cultures

1 Nov 1999

Abstract excerpt

Nuclear transfer was used to evaluate the developmental potential of nuclei from a spontaneously immortalized bovine mammary gland epithelial cell line (MECL) and from primary cultures of mammary gland cells (PMGC) and ear skin fibroblasts (PESF) established from 3-year-old cows. Cell proliferation was investigated by incorporation and detection of 5-bromo-2'-deoxyuridine (BrdU). The proportion of cells in...

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PMID: 10497348
DOI: 10.1002/(sici)1098-2795(199911)54:3<264::aid-mrd7>3.0.co;2-y

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