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Article

Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants.

European journal of biochemistry - 1 Feb 2003

Pattanaik Priyaranjan, Ravindra Gudihal, Sengupta Chandana, Maithal Kapil, Balaram Padmanabhan, Balaram Hemalatha

Abstract excerpt

Plasmodium falciparum triosephosphate isomerase (PfTIM) contains two tryptophan residues, W11 and W168. One is positioned in the interior of the protein, and the other is located on the active-site loop 6. Two single-tryptophan mutants, W11F and W168F, were constructed to evaluate the contributions of each chromophore to the fluorescence of the wild-type (wt) protein and to probe the utility of the residues as...

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PMID: 12581214
DOI: 10.1046/j.1432-1033.2003.03436.x

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