Article
NADPH-sulfite reductase from Escherichia coli. A flavin reductase participating in the generation of the free radical of ribonucleotide reductase.
The Journal of biological chemistry - 5 Sept 1993
Covès J, Nivière V, Eschenbrenner M, Fontecave M
Abstract excerpt
Protein R2, the small subunit of ribonucleotide reductase of Escherichia coli, contains an essential free radical localized to tyrosine 122 of its polypeptide chain. When this radical is scavenged by hydroxyurea, the enzyme is transformed into an inactive form, metR2. E. coli contains a NAD(P)H:f...
Topics
- Amino Acid Sequence
- Blotting, Western
- Catalysis
- Electron Transport
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- FMN Reductase
- Flavin Mononucleotide
- Flavin-Adenine Dinucleotide
- Free Radicals
- Hydroxyurea
- Molecular Sequence Data
- Molecular Weight
- Mutation
- NADH, NADPH Oxidoreductases
- NADP
- Oxidoreductases Acting on Sulfur Group Donors
- Ribonucleotide Reductases