Article
Efficient initiation of HIV-1 reverse transcription in vitro. Requirement for RNA sequences downstream of the primer binding site abrogated by nucleocapsid protein-dependent primer-template interactions.
The Journal of biological chemistry - 18 Apr 2003
Iwatani Yasumasa, Rosen Abbey E, Guo Jianhui, Musier-Forsyth Karin, Levin Judith G
Abstract excerpt
Synthesis of HIV-1 (-) strong-stop DNA is initiated following annealing of the 3' 18 nucleotides (nt) of tRNA(3)(Lys) to the primer binding site (PBS) near the 5' terminus of viral RNA. Here, we have investigated whether sequences downstream of the PBS play a role in promoting efficient (-) strong-stop DNA synthesis. Our findings demonstrate a template requirement for at least 24 bases downstream of the PBS when...
Topics
- Base Sequence
- Binding Sites
- Capsid
- Circular Dichroism
- DNA Primers
- DNA, Single-Stranded
- HIV Reverse Transcriptase
- HIV-1
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleocapsid Proteins
- Plasmids