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Article

Allele-specific long-range PCR/sequencing method for allelic assignment of multiple single nucleotide polymorphisms.

Journal of biochemical and biophysical methods - 31 Jan 2003

Nagano Michiyo, Nakamura Takahiro, Ozawa Shogo, Maekawa Keiko, Saito Yoshiro, Sawada Jun-ichi

Abstract excerpt

We report an allele-specific sequencing method using allele-specific long-range polymerase chain reaction (PCR) to determine if multiple (specifically, more than three) single nucleotide polymorphisms (SNPs) are located on the same allele. We sequenced the glucocorticoid receptor (GR) gene as a model and detected four nucleotide changes, including two novel variations, in intron 4 and exons 6, 8, and 9 alpha in...

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PMID: 12559584
DOI: 10.1016/s0165-022x(02)00114-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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