Article
Identification and characterization of glucocorticoid receptor-binding sites in the human genome.
Journal of receptor and signal transduction research - 1 Apr 2010
Taniguchi-Yanai Keiko, Koike Yoshiko, Hasegawa Takashi, Furuta Yuichi, Serizawa Masakuni, Ohshima Noriko, Kato Norihiro, Yanai Kazuyuki
Abstract excerpt
Glucocorticoids regulate gene expression via binding of the ligand-activated glucocorticoid receptor (GR) to glucocorticoid-responsive elements (GRE). To identify GR-binding sites, we developed a modified yeast one-hybrid system which enables rapid and efficient identification of genomic targets for DNA-binding proteins. The human GR expression vector was transformed into yeast cells containing a library of human...
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