Article
Direct detection of single nucleotide polymorphism (SNP) with genomic DNA by the ferrocenylnaphthalene diimide-based electrochemical hybridization assay (FND-EHA).
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Jan 2003
Nojima Takahiko, Yamashita Kenichi, Takagi Atsuko, Takagi Makoto, Ikeda Yasuyuki, Kondo Hiroki, Takenaka Shigeori
Abstract excerpt
A ferrocenylnaphthalene diimide-based electrochemical hybridization assay (FND-EHA) was applied to the direct detection of a C-to-G transition in a codon (TCA) for Ser-447 of the human lipoprotein lipase (LPL) gene, which resulted in the termination of the LPL protein there. Either one of two 13-meric oligonucleotide probes, S447 WT and S447X MT, representing sequences complementary to those of the wild type (WT)...
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