Article
Electrochemical SNP detection.
Nucleic acids symposium series (2004) - 1 Jan 2006
Watanabe Sadayoshi, Mukumoto Kosuke, Nojima Takahiko, Waki Michinori, Takenaka Shigeori
Abstract excerpt
Detection of mismatched base on a DNA duplex, which is important for the search of the heterozygote, achieved by the comparison with the reactivity of ferrocenylcarbodiimide (1) developed by our group. Mismatched thymine base on the 30-meric DNA duplex as a model of SNPs in lipoprotein lipase (LPL) gene, could react with 1 in 20 mM borate buffer (pH 8.5) and 0.1 M NaCl containing 20% DMSO at 37 degrees C. DNA...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
