Article
Structural evidence for substrate strain in antibody catalysis.
Proceedings of the National Academy of Sciences of the United States of America - 4 Feb 2003
Yin Jun, Andryski Scott E, Beuscher Albert E, Stevens Raymond C, Schultz Peter G
Abstract excerpt
The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The...
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