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Article

Porphyrin-substrate binding to murine ferrochelatase: effect on the thermal stability of the enzyme.

The Biochemical journal - 15 Mar 2005

Franco Ricardo, Bai Guangyue, Prosinecki Vesna, Abrunhosa Filipa, Ferreira Gloria C, Bastos Margarida

Abstract excerpt

Ferrochelatase (EC 4.99.1.1), the terminal enzyme of the haem biosynthetic pathway, catalyses the chelation of Fe(II) into the protoporphyrin IX ring. The energetics of the binding between murine ferrochelatase and mesoporphyrin were determined using isothermal titration calorimetry, which revealed a stoichiometry of one molecule of mesoporphyrin bound per protein monomer. The binding is strongly exothermic, with...

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Identifiers

PMID: 15496139
DOI: 10.1042/BJ20040921
PMCID: PMC1134880

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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