Article
Limited diversity of the immunoglobulin heavy chain variable domain of the emerald rockcod Trematomus bernacchii.
Fish & shellfish immunology - 1 Jan 2003
Coscia Maria Rosaria, Oreste Umberto
Abstract excerpt
To investigate the diversity of the immunoglobulin heavy chain variable domain of the cold adapted teleost Trematomus bernacchii, 45 cDNA clones, containing complete or partial sequences of rearranged VH/D/JH segments, were analysed. Clones were isolated from a spleen library constructed by 5' RACE or from an expression library previously constructed and immunoscreened with rabbit anti- T. bernacchii Ig heavy...
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