Article
Human mismatch-repair protein MutL homologue 1 (MLH1) interacts with Escherichia coli MutL and MutS in vivo and in vitro: a simple genetic system to assay MLH1 function.
The Biochemical journal - 1 Apr 2003
Quaresima Barbara, Alifano Pietro, Tassone Pierfrancesco, Avvedimento Enrico V, Costanzo Francesco S, Venuta Salvatore
Abstract excerpt
A simple genetic system has been developed to test the effect of over-expression of wild-type or mutated human MutL homologue 1 (hMLH1) proteins on methyl-directed mismatch repair (MMR) in Escherichia coli. The system relies on detection of Lac(+) revertants using MMR-proficient or MMR-deficient E. coli strains carrying a lac +1 frameshift mutation expressing hMLH1 proteins. We report that expression of wild-type...
Topics
- Adaptor Proteins, Signal Transducing
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Alleles
- Bacterial Proteins
- Base Pair Mismatch
- Carrier Proteins
- Colorectal Neoplasms, Hereditary Nonpolyposis
- DNA Repair
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins