Article
Trafficking of mutant carboxypeptidase E to secretory granules in a beta-cell line derived from Cpe(fat)/Cpe(fat) mice.
Endocrinology - 1 Jan 2003
Cawley Niamh X, Rodriguez Yazmin M, Maldonado Alex, Loh Y Peng
Abstract excerpt
We have reinvestigated the stability and intracellular routing of mutant carboxypeptidase E in NIT3 cells, a pancreatic beta-cell line derived from the Cpe(fat)/Cpe(fat) mouse. Pulse-chase experiments demonstrated that this protein has a half-life of approximately 3 h in these cells and that up to 45% of the proCPE(202) can escape degradation by the proteosome. In double-label immunofluorescence microscopy, a...
Topics
- Animals
- Blotting, Western
- Carboxypeptidase H
- Carboxypeptidases
- Cell Line
- Enzyme Precursors
- Enzyme Stability
- Fluorescent Antibody Technique, Indirect
- Glucagon
- Glucagon-Like Peptide 1
- Half-Life
- Islets of Langerhans
- Mice
- Microscopy, Fluorescence
- Mutation
- Peptide Fragments
- Proprotein Convertase 2
- Protein Precursors