Article
[Identification of AP1 cis-element and transcriptional effect on cytokeratin 13 gene expression].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Dec 2002
Lin Gongbiao, Xiao Jianyun, Zhao Suping, Tian Yongquan, Wang Chenglong, Qiu Yuanzheng
Abstract excerpt
OBJECTIVE: To identify the type of CTGAATCA from -nt.199 to -nt.192 of the cytokeratin 13(CK13) gene 5' flanking region and determine its transcriptional effect on CK13 gene expression. METHODS: The CAT systems were used to assess the effects of different motifs of CK13 gene 5' flanking region on transcription. The clones of pCAT-enhancer with the total length, -nt.207 to +nt.63 and the same length of -nt.207 to...
Topics
- 5' Flanking Region
- Base Sequence
- Binding Sites
- Binding, Competitive
- Chloramphenicol O-Acetyltransferase
- DNA
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation
- HeLa Cells
- Humans
- Keratins
- Mutation
- Recombinant Fusion Proteins