Article
DMSO-treated HL60 cells: a model of neutrophil-like cells mainly expressing PDE4B subtype.
International immunopharmacology - 1 Nov 2002
Jacob C, Leport M, Szilagyi C, Allen J M, Bertrand C, Lagente V
Abstract excerpt
The human promyelocytic HL60 cells acquired a neutrophilic phenotype after a 7- to 10-day DMSO treatment. Fc gammaRII was up-regulated. Fc gammaRI was also up-regulated by an additional IFN-gamma treatment. These cells are able to produce O2*- by NADPH oxidase activation in the presence of immune complexes or phorbol-12-myristate-13-acetate (PMA). A change of their PDE4 subtype profile was also observed: PDE4B...
Topics
- 3',5'-Cyclic-AMP Phosphodiesterases
- Cell Differentiation
- Cell Size
- Cyclic Nucleotide Phosphodiesterases, Type 3
- Cyclic Nucleotide Phosphodiesterases, Type 4
- Dimethyl Sulfoxide
- Down-Regulation
- Enzyme Inhibitors
- Flow Cytometry
- Gene Expression