Article

AN OPTIMIZED METHOD TO DIFFERENTIATE HL60 CELLS INTO NEUTROPHIL-LIKE CELLS

2026-01-08

Abstract excerpt

<h4>ABSTRACT</h4> The HL60 promyelocytic leukemia cell line is widely used to investigate neutrophil biology due to its genetic tractability and accessibility. HL60 cells can be differentiated into neutrophil-like cells using all-trans retinoic acid (ATRA) or dimethyl sulfoxide (DMSO) treatment. However, these approaches produce cells that lack critical features of mature granulocytes, such as robust chemotaxis w...

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Literature Corpus work: 12c6c814-6c54-5f0b-9c62-7c5272d56b76
DOI: 10.64898/2026.01.06.697988

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