Article
Absence of both subunits of cytochrome b558 in the UM384 cell line relative to the inability to generate superoxide anions.
Experimental hematology - 1 Jul 1993
Champelovier P, Laporte F, Verhoeven A J, Hilarius P, de Klein A, Revol C, Seigneurin D, Kolodie L
Abstract excerpt
Phagocytic cells are characterized by their ability to generate superoxide anions upon activation by appropriate stimuli. UM384, a myelomonocytic cell line, was shown to be defective in this oxidase activity as measured by nitroblue tetrazolium or cytochrome c reduction. Cytochrome b558, a unique...
Topics
- Antibodies, Monoclonal
- Blotting, Western
- Cell Differentiation
- Cell Line
- Cytochrome b Group
- Cytochrome c Group
- Humans
- Immunohistochemistry
- Monocytes
- NADH, NADPH Oxidoreductases
- NADPH Oxidases
- Neutrophils
- Nitroblue Tetrazolium
- Oxidation-Reduction
- Phagocytes
- Phenotype
- RNA, Messenger
- Superoxides