Estrogen regulation of transferrin gene expression in MCF-7 human breast cancer cells.

Abstract excerpt

Transferrin (Tf) is an iron transport protein expressed in MCF-7 human breast cancer cells. In nuclear run-on assays, 17beta-estradiol (E2) increased the rate of Tf gene expression approximately 3-fold within 1 h after treatment and reporter gene activity was also induced in MCF-7 cells transfected with a construct containing a -3600 to +39 Tf gene promoter insert. Deletion and mutation analysis identified an...

Topics

• Breast Neoplasms
• Electrophoretic Mobility Shift Assay
• Estrogen Receptor alpha
• Estrogens
• Gene Expression Regulation, Neoplastic
• Humans
• Mutation
• Promoter Regions, Genetic
• Receptors, Estrogen
• Response Elements
• Transfection