Article
High performance DNA sequencing, and the detection of mutations and polymorphisms, on the Clipper sequencer.
Electrophoresis - 1 Jun 1999
Yager T D, Baron L, Batra R, Bouevitch A, Chan D, Chan K, Darasch S, Gilchrist R, Izmailov A, Lacroix J M, Marchelleta K, Renfrew J, Renfrew J, Rushlow D, Steinbach E, Ton C, Waterhouse P, Zaleski H, Dunn J M, Stevens J
Abstract excerpt
The Visible Genetics Clipper sequencer is a new platform for automated DNA sequencing which employs disposable MicroCel cassettes and 50 microm thick polyacrylamide gels. Two DNA ladders can be analyzed simultaneously in each of 16 lanes on a gel, after labeling with far-red absorbing dyes such as Cy5 and Cy5.5. This allows a simultaneous bidirectional sequencing of four templates. We have evaluated the Clipper...
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