Article
Key amino acids for differential coupling of alpha1-adrenergic receptor subtypes to Gs.
Biochemical and biophysical research communications - 22 Nov 2002
Shinoura Hitomi, Shibata Katsushi, Hirasawa Akira, Tanoue Akito, Hashimoto Keitaro, Tsujimoto Gozoh
Abstract excerpt
We have established that differing effects of alpha1-adrenergic receptor (AR) subtypes on cell proliferation are due to differential coupling to the Gs/cAMP pathway; thus, both alpha1A- and alpha1B-ARs couple to Gs, while alpha1D-AR does not. To identify the region responsible for this difference in subtype-specific Gs coupling, we constructed a series of chimeric and a set of point-mutated human alpha1A- and...
Topics
- Amino Acid Sequence
- Amino Acids
- Cell Division
- Cell Membrane
- Cyclic AMP
- Humans
- Ligands
- Molecular Sequence Data
- Mutation
- Protein Binding
- Radioligand Assay
- Receptors, Adrenergic, alpha-1
- Sequence Homology, Amino Acid
- Signal Transduction
- Threonine
- Transfection
- Valine