Article
[Relationship between the different replication status of HBV and mutations in core promoter in mother and children infected by mother-to-infant transmission].
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology - 1 Oct 2002
Xu Hongmei, Ren Hong, Qing Yuling, Peng Mingli, Ling Ning
Abstract excerpt
OBJECTIVE: To understand the relationship between the different replication status of HBV and mutations in core promoter in mother and child infected by mother-to-infant transmission. METHODS: Core promoter was amplified by PCR and cloned into pGEM-T vector with T-A cloning technique. The recombinant plasmid pGEM-PreS/S was confirmed by digestion with restriction enzyme Apa I and Sac I. Two clones were selected...
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