Article
Functional expression of Acetabularia acetabulum vacuolar H(+)-pyrophosphatase in a yeast VMA3-deficient strain.
Journal of experimental botany - 1 Nov 2002
Ikeda Mikiko, Umami Kimiko, Hinohara Misato, Tanimura Yoshie, Ohmae Atsuko, Nakanishi Yoichi, Maeshima Masayoshi
Abstract excerpt
The function of the translation product of cDNA for Acetabularia vacuolar H(+)-pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3-deficient strain. The open reading frame of Acetabularia H(+)-pyrophosphatase was revealed to encode 751 amino acids (721 or 751 amino acids in a previous paper). The acidification of the vacuole was observed by fluorescence microscopy when the cDNA was constructed in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
