Article
Limitations on the recombinant plasmid selection by Lac(+)/Lac(-) colony phenotype detection.
Biochemical and biophysical research communications - 18 Oct 2002
Gutkina Nadezhda, Varlakhanova Natalia V, Lysova Marina V, Kovalenko Sergei P
Abstract excerpt
Lac(+)/Lac(-) selection of recombinant plasmids based on the insertional inactivation of LacZalpha gene cannot differentiate recombinant clones in some cases. Several fragments of exon 11 of human brca1 gene were cloned in LacZalpha-containing plasmids so that frameshift appeared at the 5(')-end of the fragments tested but these fragments were in frame with the part of LacZalpha situated downstream of the...
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