Problems encountered in detecting a targeted gene by the polymerase chain reaction.

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Problems encountered in detecting a targeted gene by the polymerase chain reaction.

Gene - 22 Jul 1991

Kim H S, Popovich B W, Shehee W R, Shesely E G, Smithies O

Abstract excerpt

We have investigated problems encountered when using the polymerase chain reaction (PCR) to detect recombinants in gene targeting experiments in which homologous recombination occurs between incoming DNA and an endogenous target sequence. The targeting system studied was designed to correct a hum...

Topics

Animals
Base Sequence
Blotting, Southern
Cell Nucleus
Chromosomes, Human, Pair 11
Electric Stimulation
False Positive Reactions
Genetic Therapy
Globins
Humans
Mice
Microinjections
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Recombination, Genetic
Sickle Cell Trait
Tumor Cells, Cultured

Source

Open in PubMed

Identifiers

PMID: 1889748

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1889748-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics