Article
Problems encountered in detecting a targeted gene by the polymerase chain reaction.
Gene - 22 Jul 1991
Kim H S, Popovich B W, Shehee W R, Shesely E G, Smithies O
Abstract excerpt
We have investigated problems encountered when using the polymerase chain reaction (PCR) to detect recombinants in gene targeting experiments in which homologous recombination occurs between incoming DNA and an endogenous target sequence. The targeting system studied was designed to correct a human sickle-cell beta-globin-encoding gene (HBBS) on human chromosome 11 by replacing the defective gene with incoming...
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