Article
Engineered viruses to select genes encoding secreted and membrane-bound proteins in mammalian cells.
Nucleic acids research - 1 Oct 2002
Moffatt Pierre, Salois Patrick, Gaumond Marie-Hélène, St-Amant Natalie, Godin Eric, Lanctôt Christian
Abstract excerpt
We have developed a functional genomics tool to identify the subset of cDNAs encoding secreted and membrane-bound proteins within a library (the 'secretome'). A Sindbis virus replicon was engineered such that the envelope protein precursor no longer enters the secretory pathway. cDNA fragments were fused to the mutant precursor and expression screened for their ability to restore membrane localization of envelope...
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