Article
Studies on the function of oligosaccharyl transferase subunits. Stt3p is directly involved in the glycosylation process.
The Journal of biological chemistry - 6 Dec 2002
Yan Qi, Lennarz William J
Abstract excerpt
In the yeast, Saccharomyces cerevisiae, oligosaccharyl transferase (OT) is composed of nine different transmembrane proteins. Using a glycosylatable peptide containing a photoprobe, we previously found that only one essential subunit, Ost1p, was specifically labeled by the photoprobe and recently have shown that it does not contain the recognition domain for the glycosylatable sequence Asn-Xaa-Thr/Ser. In this...
Topics
- Amino Acid Sequence
- Catalytic Domain
- Dose-Response Relationship, Drug
- Glycoside Hydrolases
- Glycosylation
- Hexosyltransferases
- Membrane Proteins
- Models, Biological
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Phenotype
- Plasmids
- Point Mutation