Article
A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro.
The EMBO journal - 17 Mar 1997
Reiss G, te Heesen S, Gilmore R, Zufferey R, Aebi M
Abstract excerpt
The central reaction in the process of N-linked protein glycosylation in eukaryotic cells, the transfer of the oligosaccharide Glc(3)Man(9)GlcNAc(2) from the lipid dolicholpyrophosphate to selected asparagine residues, is catalyzed by the oligosaccharyltransferase (OTase). This enzyme consists of...
Topics
- Amino Acid Sequence
- Base Sequence
- Carbohydrate Sequence
- Chromosome Mapping
- DNA Primers
- DNA, Fungal
- Gene Expression
- Genes, Fungal
- Glycosylation
- Hexosyltransferases
- Introns
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Oligosaccharides