Severe IgE‐mediated anaphylaxis following consumption of fried frog legs: definition of α‐parvalbumin as the allergen in cause

Abstract excerpt

BACKGROUND: IgE-mediated allergic reactions to bullfrog and edible frog have been reported. The implicated allergens have not been defined so far. The frog material and the patient's serum from a case of severe food-induced anaphylaxis were used to define the implicated allergen at the protein and DNA level. METHODS: Immunoblotting techniques and N-terminal protein microsequencing were used to define the allergen...

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