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Article

A ribosomal RNA gene intergenic spacer based PCR and DGGE fingerprinting method for the analysis of specific rhizobial communities in soil.

Journal of microbiological methods - 1 Mar 2006

de Oliveira Valéria Maia, Manfio Gilson Paulo, da Costa Coutinho Heitor Luiz, Keijzer-Wolters Anneke Christina, van Elsas Jan Dirk

Abstract excerpt

A direct molecular method for assessing the diversity of specific populations of rhizobia in soil, based on nested PCR amplification of 16S-23S ribosomal RNA gene (rDNA) intergenic spacer (IGS) sequences, was developed. Initial generic amplification of bacterial rDNA IGS sequences from soil DNA was followed by specific amplification of (1) sequences affiliated with Rhizobium leguminosarum "sensu lato" and (2) R....

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Identifiers

PMID: 16014316
DOI: 10.1016/j.mimet.2005.05.015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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