Sequence analysis of rpoB and rpoD gene fragments reveals the phylogenetic diversity of actinobacteria of genus Frankia.

doi:10.1139/W10-106

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Article

Sequence analysis of rpoB and rpoD gene fragments reveals the phylogenetic diversity of actinobacteria of genus Frankia.

Canadian journal of microbiology - 1 Mar 2011

Bernèche-D'Amours Audrey, Ghinet Mariana Gabriela, Beaudin Julie, Brzezinski Ryszard, Roy Sébastien

Abstract excerpt

Partial rpoD, rpoB, and 16S rRNA gene sequences were obtained from databases and (or) amplified from 12 strains of Frankia. These strains belonged to either Cluster 1 (Alnus-, Myrica-, Comptonia-, and Casuarina-infective strains) or Cluster 3 (Elaeagnus-infective strain). An rpoD gene-based PCR approach was designed to allow the detection of frankiae in complex samples. Additionally, partial gene sequences...

Topics

Bacterial Proteins
DNA, Bacterial
DNA-Directed RNA Polymerases
Frankia
Genes, Bacterial
Genetic Variation
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Sequence Alignment
Sequence Analysis, DNA
Sigma Factor

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Open in PubMed

Identifiers

PMID: 21358766
DOI: 10.1139/W10-106

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