Article
Preparation of homogeneous samples of double-labelled protein suitable for single-molecule FRET measurements.
Analytical and bioanalytical chemistry - 1 Jul 2013
Lerner E, Hilzenrat G, Amir D, Tauber E, Garini Y, Haas E
Abstract excerpt
Preparation of pure and homogenous site specifically single- and double-labelled biopolymers suitable for spectroscopic determination of structural characteristics is a major current challenge in biopolymers chemistry. In particular, proper analysis of single-molecule Förster resonance energy transfer measurements is based on the spectral characteristics of the probes. Heterogeneity of any of the probes may...
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