Article
Enhanced β-galactosidase production from whey powder by a mutant of the psychrotolerant yeast Guehomyces pullulans 17-1 for hydrolysis of lactose.
Applied biochemistry and biotechnology - 1 Feb 2012
Xu Jin-Li, Zhao Jun, Wang Ling-Fei, Sun Huai-Yong, Song Chun-Li, Chi Zhen-Ming
Abstract excerpt
In order to isolate β-galactosidase overproducers of the psychrotolerant yeast Guehomyces pullulans 17-1, its cells were mutated by using nitrosoguanidine (NTG). One mutant (NTG-133) with enhanced β-galactosidase production was obtained. The mutant grown in the production medium with 30.0 g/l lactose and 2.0 g/l glucose could produce more β-galactosidase than the same mutant grown in the production medium with...
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