Article
Studies on the escape mutants of rabies virus which are resistant to neutralization by a highly conserved conformational epitope-specific monoclonal antibody #1-46-12.
Microbiology and immunology - 1 Jan 2002
Irie Takashi, Matsuda Yukihiro, Honda Yoshikazu, Morimoto Kinjiro, Kawai Akihiko
Abstract excerpt
We investigated a virus-neutralizing conformational epitope of the rabies virus glycoprotein (G) that is recognized by an anti-G monoclonal antibody (mAb; #1-46-12) and shared by most of the laboratory strains of the virus. To investigate the epitope structure, we isolated escape mutants from the HEP-Flury virus (wild-type; wt) after repeated passages in culture in the presence of the mAb. Immunofluorescence...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibodies, Viral
- Antigens, Viral
- Base Sequence
- Cells, Cultured
- Electrophoresis, Polyacrylamide Gel
- Epitopes
- Evolution, Molecular
- Fluorescent Antibody Technique
- Glycoproteins
- Models, Genetic