Isolation and characterization of large numbers of endothelial cells for studies of cell signaling.

Abstract excerpt

Studies of endothelial cell signaling involving cholesterol-rich domains require large numbers of cells of bona fide origin. The growth of any cell in culture, particularly for extended periods, results in an altered phenotype that could include changes in the properties of caveolae and lipid raft structures. While continuously propagated cells are used to study specific questions because their origin is known...

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