Article
Establishment and long-term culture of human cystic fibrosis endothelial cells.
Laboratory investigation; a journal of technical methods and pathology - 1 Nov 2017
Plebani Roberto, Tripaldi Romina, Lanuti Paola, Recchiuti Antonio, Patruno Sara, Di Silvestre Sara, Simeone Pasquale, Anile Marco, Venuta Federico, Prioletta Marco, Mucilli Felice, Del Porto Paola, Marchisio Marco, Pandolfi Assunta, Romano Mario
Abstract excerpt
Endothelial cell (EC) dysfunction has been reported in cystic fibrosis (CF) patients. Thus, the availability of CF EC is paramount to uncover mechanisms of endothelial dysfunction in CF. Using collagenase digestion, we isolated cells from small fragments of pulmonary artery dissected from non-CF lobes or explanted CF lungs. These cells were a heterogeneous mixture, containing variable percentages of EC. To obtain...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.