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Article

Isolation and short-term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study.

Microcirculation (New York, N.Y. : 1994) - 1 Apr 2023

Jablon Kelli L, Akerstrom Victoria L, Li Min, Braun Stephen E, Norton Charles E, Castorena-Gonzalez Jorge A

Abstract excerpt

OBJECTIVE: To develop an experimental method for routine isolation and short-term culture of primary lymphatic endothelial cells from specific collecting vessels. METHODS: Lymphatic endothelial cell tubes (LECTs) were isolated from micro-dissected collecting vessels. LECTs were allowed to attach and grow for ~3 weeks before being passaged. Non-purified cultures were partially characterized by immunofluorescence...

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PMID: 35879879
DOI: 10.1111/micc.12778
PMCID: PMC9873843

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