Article
Quantity of mutant K-ras gene in pancreatic secretions for diagnosis of pancreatic carcinoma with different assays: analysis of 100 patients.
Clinica chimica acta; international journal of clinical chemistry - 1 Oct 2002
Tada Minoru, Tateishi Keisuke, Kawabe Takao, Sasahira Naoki, Isayama Hiroyuki, Komatsu Yutaka, Shiratori Yasushi, Omata Masao
Abstract excerpt
BACKGROUND: Two types of quantitative assay kits for K-ras mutations, PCR-preferential homoduplex formation assay (PHFA) and enriched PCR and enzyme-linked mini-sequence assay (ELMA), were recently developed. The K-ras mutations were analyzed using these assays. MATERIALS AND METHODS: DNA was extracted from the pancreatic juice which was obtained by endoscopy from 38 patients with pancreatic neoplasms (23...
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