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[Overexpression and detection of the mutated glucose isomerase GIG138P and GIG138P-G247D in Streptomyces lividans].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 May 2002

Zhu Guo-Ping, Zhang Ying, Xu Yang, Tang Jian-Guo, Xu Chong

Abstract excerpt

The shuttle expression vectors pHZGI1 and pHZGI2 were successfully constructed by inserting structural genes of GI containing single mutated site G138P and double mutated site G138P-G247D into E. coli-Streptomyces shuttle vector pHZ-1272, respectively. Then they were transformed into S. lividans TK54 strain by protoplast transformation. SDS-PAGE indicated that two shuttle vectors in TK54 strain expressed...

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PMID: 12192862

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