Article
Construction of double-copy glucose isomerase gene engineering strain of Streptomyces diastaticus by homologous recombination.
Current microbiology - 1 Apr 2002
Zhang Ying, Xu Chong, Lu Zhiqiang, Yang Yonghui, Ge Feng, Zhu Guoping, Teng Maikun, Niu Liwen
Abstract excerpt
The plasmid pUT for homologous recombination was constructed by the insertion of the 1.1-kb thiostrepton resistance ( tsr(R)) gene into the E. coli plasmid pUB1-GI1. Plasmid pUTK was produced through ligating the cleaved plasmid pUT by KpnI. After pUT and pUTK were introduced into Streptomyces diastaticus No.7 strain M1033 (SM33) by protoplast transformation, a series of tsr(R) transformants were obtained,...
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