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Article

Site-specific integration of the double-mutation glucose isomerase (GIG138PG247D) gene in Streptomyces lividans and its stable expression.

Current microbiology - 1 Jan 2002

Yang Yonghui, Xu Chong, Ge Feng, Lu Zhiqiang, Zhu Guoping, Li Hui, Liao Jun, Niu Liwen, Wang Yuzhen, Wu Mian

Abstract excerpt

A recombinant expression plasmid pYH12, containing the double-mutation glucose isomerase (GIG138PG247D, GI2) coding gene and its natural regulatory sequence, was constructed for site-specific integration in Streptomyces. The resulting plasmid was introduced into Streptomyces lividans TK54 by protoplast transformation and two apramycin-resistance (AmR) transformants, designated GY2 and BY7, respectively, were...

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PMID: 11727036
DOI: 10.1007/s00284-001-0068-7

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