Article
Glucose isomerase of the Streptomyces sp. SK strain: purification, sequence analysis and implication of alanine 103 residue in the enzyme thermostability and acidotolerance.
Biochimie - 1 Aug 2004
Borgi Mohamed Ali, Srih-Belguith Karima, Ben Ali Mamdouh, Mezghani Monia, Tranier Samuel, Haser Richard, Bejar Samir
Abstract excerpt
The glucose isomerase gene (xylA) from the Streptomyces sp. SK strain encodes a 386-amino-acid protein (42.7 kDa) showing extensive identities with many other bacterial glucose isomerases. We have shown by gel filtration chromatography and SDS-PAGE analysis that the purified recombinant glucose isomerase (SKGI) is a 180 kDa tetramer of four 43 kDa subunits. Sequence inspection revealed that this protein, present...
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