Article
IGF-I binding in primary culture of muscle cells of rainbow trout: changes during in vitro development.
American journal of physiology. Regulatory, integrative and comparative physiology - 1 Sept 2002
Castillo Juan, Le Bail Pierre-Yves, Paboeuf Gilles, Navarro Isabel, Weil Claudine, Fauconneau Benoit, Gutiérrez Joaquim
Abstract excerpt
To characterize and study the variations of IGF-I binding during the development of trout muscle cells, in vitro experiments were conducted using myocyte cultures, and IGF-I binding assays were performed in three stages of cell development: mononuclear cells (day 1), small myotubes (day 4), and large myotubes (day 10). Binding experiments were done by incubating cells with IGF-I for 12 h at 4 degrees C. Specific...
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