Article
Analysis of length variation in the V1-V2 region of env in nonsubtype B HIV type 1 from Uganda.
AIDS research and human retroviruses - 20 Jul 2002
Klevytska Alexandra M, Mracna Martin R, Guay Laura, Becker-Pergola Graziella, Furtado Manohar, Zhang Linqi, Jackson J Brooks, Eshleman Susan H
Abstract excerpt
We optimized an assay for analysis of length variation in the V1-V2 region of HIV-1 env in plasma samples from Uganda. V1-V2 env length variation was analyzed in 31 plasma samples containing subtype A, C, D, or A/D recombinant HIV-1. DNA corresponding to the V1-V2 region was amplified by nested PCR. One of the primers in the second step of the PCR was fluorescently labeled. Successful amplification was confirmed...
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