Article
Determination of human beta(2)-adrenoceptor haplotypes by denaturation selective amplification and subtractive genotyping.
American journal of pharmacogenomics : genomics-related research in drug development and clinical practice - 1 Jan 2001
Wang J, Mougey E B, David C J, Humma L M, Johnson J A, Lima J J, Sylvester J E
Abstract excerpt
OBJECTIVE: beta(2)-Adrenoceptor haplotype may be better associated with asthma severity and drug response than a polymorphic variant at any single site. Because present methods of haplotype determination are time consuming and impractical for large population studies, we sought to develop a simple and efficient method of determining haplotype of 3 common polymorphisms at codons -19 (Arg/Cys), 16 (Arg/Gly) and 27...
Topics
- DNA Primers
- Genotype
- Haplotypes
- Humans
- Nucleic Acid Amplification Techniques
- Nucleic Acid Denaturation
- Polymorphism, Restriction Fragment Length
- Receptors, Adrenergic, beta-2