Article
The effect of core destabilization on the mechanical resistance of I27.
Biophysical journal - 1 Jul 2002
Brockwell David J, Beddard Godfrey S, Clarkson John, Zinober Rebecca C, Blake Anthony W, Trinick John, Olmsted Peter D, Smith D Alastair, Radford Sheena E
Abstract excerpt
It is still unclear whether mechanical unfolding probes the same pathways as chemical denaturation. To address this point, we have constructed a concatamer of five mutant I27 domains (denoted (I27)(5)*) and used it for mechanical unfolding studies. This protein consists of four copies of the mutant C47S, C63S I27 and a single copy of C63S I27. These mutations severely destabilize I27 (DeltaDeltaG(UN) = 8.7 and...
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