Article
Structural elements that govern the substrate specificity of the clot-dissolving enzyme plasmin.
The Journal of biological chemistry - 6 Sept 2002
Turner Ryan B, Liu Lin, Sazonova Irina Y, Reed Guy L
Abstract excerpt
There is remarkable homology between the core structures of plasmin, a fibrin clot-degrading enzyme, and factor D, a complement-activating enzyme, despite markedly different biological functions. We postulated that sequence divergence in the loop structures between these two enzymes mediated the unique substrate and inhibitor interactions of plasmin. Recombinant microplasminogens chimerized with factor D...
Topics
- Binding Sites
- Catalysis
- Cloning, Molecular
- Complement Factor D
- Dose-Response Relationship, Drug
- Fibrin
- Fibrinogen
- Fibrinolysin
- Fibrinolytic Agents
- Humans
- Kinetics
- Models, Molecular
- Mutation
- Plasminogen
- Protein Binding