Article
Evaluation of a commercial loop-mediated isothermal amplification assay for diagnosis of Bordetella pertussis infection.
Journal of microbiological methods - 1 Feb 2017
Kamachi Kazunari, Moriuchi Takumi, Hiramatsu Yukihiro, Otsuka Nao, Shibayama Keigo
Abstract excerpt
We evaluated a commercial loop-mediated isothermal amplification (LAMP) assay kit for Bordetella pertussis detection. The LAMP primers were designed to target the ptxP1 allele of the pertussis toxin promoter, but the assay could detect B. pertussis ptxP3 and ptxP8 strains in addition to ptxP1 strains, with high analytical sensitivity.
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