Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Novel approach for linking genotype to phenotype in vitro by exploiting an extremely strong interaction between RNA and protein.

Journal of medicinal chemistry - 11 Apr 2002

Fujita Satoshi, Sawata Shinya Y, Yamamoto-Fujita Rika, Endo Yaeta, Kise Hideo, Iwakura Masahiro, Taira Kazunari

Abstract excerpt

We recently isolated an aptamer that binds to the Tat protein of HIV-1 with extremely high affinity and specificity (Yamamoto, R.; et al. Genes Cells 2000, 5, 371.). In the present study, we exploited this strong binding to develop a novel coupling method that links genotype with phenotype. To strengthen the original RNA-protein interaction still further, we connected three units of the aptamer in tandem and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Codon, Terminator
Share to Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Products, tat

Share to Protein Biosynthesis

Share to Ribosomes

Share to Streptavidin

Share to Tetrahydrofolate Dehydrogenase

Source

Identifiers

PMID: 11931614
DOI: 10.1021/jm010398p

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 11 closest

Novel approach for linking genotype to phenotype in vitro by exploiting an extremely strong interaction between RNA and protein.PMID 11931614Read ↗

A novel approach to selection of functional proteins.Semantic proximity Read ↗

Photocleavable linkage between genotype and phenotype for rapid and efficient recovery of nucleic acids encoding affinity-selected proteins.Semantic proximity Read ↗

Advantages of mRNA display selections over other selection techniques for investigation of protein–protein interactionsSemantic proximity Read ↗

Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope.Semantic proximity Read ↗

Mathematical consideration of massive estimation of dissociation rate constant for genotype-phenotype linking molecules bound to targets through washing/selection and next-generation sequencing.Semantic proximity Read ↗

Fluorescence correlation spectroscopy at single molecule level on the Tat-TAR complex and its inhibitors.Semantic proximity Read ↗

Identification of amino acids that promote specific and rigid TAR RNA-tat protein complex formation.Semantic proximity Read ↗

A new plasmid display technology for the in vitro selection of functional phenotype-genotype linked proteins.Semantic proximity Read ↗

Escherichia coli Hfq binds A18 and DsrA domain II with similar 2:1 Hfq6/RNA stoichiometry using different surface sites.Semantic proximity Read ↗

Purification and functional characterization of wild-type and mutant HIV-1 and HIV-2 Tat proteins expressed in Escherichia coli.Semantic proximity Read ↗

Bimodal loop-loop interactions increase the affinity of RNA aptamers for HIV-1 RNA structures.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.