Mathematical consideration of massive estimation of dissociation rate constant for genotype-phenotype linking molecules bound to targets through washing/selection and next-generation sequencing.

Abstract excerpt

As one of methods for in vitro selection, a flow reactor type washing/selection system seems to be effective, where a ligand library is composed of "genotype-phenotype linking molecules". In this system, high affinity ligands are selected by their respective "residual ratio" given by exp(-koff×t), where koff is the dissociation rate constant and t is the washing time. In this paper, we mathematically considered...

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